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Speaker: Gayle E. Woloschak, PhD
Professor, Departments of Radiation Oncology,
Radiology, and Cell and Molecular Biology
Robert H. Lurie Cancer Center
Northwestern University, Feinberg School of Medicine
website: http://www.radiology.northwestern.edu/research/people/researcher.cfm?PersonID=796
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Topic:
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"Nanotechnology, Nanocomposites, and Cancer" |
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Experiments are being done are with titanium dioxide based nanocomposites (TiNCs) which belong to the category of bio-nanocomposites—nanometer-sized particles created by the conjugation of inorganic with “traditionally” biological molecules. These nanocomposites are prepared from metal oxide (TiO2) nanoparticles (4.5 nm in size, surface-coated with glycidyl isopropyl ether) and DNA oligonucleotides covalently bound via dopamine to the nanoparticles. Within the nanocomposites the DNA oligonucleotides retain their base-pairing specificity, while the TiO2 nanoparticles exhibit a semiconductor characteristic photoreactivity. In particular, excitation of TiO2 (induced by exposure to electromagnetic radiation of energy above 3.2 eV) results in charge separation ultimately resulting in scission of the DNA phosphodiester backbone in the adjacent DNA double
helix. This endonuclease-like activity is therefore: (a) excitable by a factor not naturally encountered by cells in vivo (electromagnetic radiation of energy higher than 3.2 eV); and (b) highly sequence specific. This cleavage can be directed toward a single target gene in a whole genome (due to the high specificity of long oligonucleotide base-pairing); consequently, TiNCs behave as inducible gene-specific endonucleases with allele-differentiating sequence specificity. Intracellular studies have demonstrated the ability to localize these nanocomposites to particular sites based on DNA sequence. They are currently being developed for imaging and therapy applications.
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When:
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Monday, December 18, 2006
12:00PM – 1:00PM
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Location:
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Baldwin Auditorium
Robert H. Lurie Medical Research Center
303 East Superior Street, 1st floor
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Selected Recent References for Gayle E. Woloschak, PhD
Rhee TK, Larson AC, Prasad PV, Santos E, Sato KT, Salem R, Deng J, Paunesku T, Woloschak GE, Mulcahy MF, Li D, Omary RA. Feasibility of blood oxygenation level-dependent MR imaging to monitor hepatic transcatheter arterial embolization in rabbits. J Vasc Interv Radiol. 2005 Nov;16(11):1523-8.
Lai B, Maser J, Vogt S, Paunesku T, Woloschak GE. Workshop on biological applications of X-ray microscopy and imaging. Advanced Photon Source, Argonne National Laboratory, Argonne, IL, USA, 28-29 April 2003. Int J Radiat Biol. 2004 Jun;80(6):459-61.
Lai B, Maser J, Paunesku T, Woloschak GE. Report on the Workshop of Biological Applications of X-ray Microbeams. Int J Radiat Biol. 2002 Aug;78(8):749-52.
Woloschak GE, Paunesku T, Protic M. Sensitivity to low-level radiation in radiosensitive "wasted" mice. Mil Med. 2002 Feb;167(2 Suppl):42-3.
Paunesku T, Mittal S, Protic M, Oryhon J, Korolev SV, Joachimiak A, Woloschak GE. Proliferating cell nuclear antigen (PCNA): ringmaster of the genome. Int J Radiat Biol. 2001 Oct;77(10):1007-21.
Watson CA, Chang-Liu CM, Woloschak GE. Modulation of calmodulin by UV and X-rays in primary human endothelial cell cultures. Int J Radiat Biol. 2000 Nov;76(11):1455-61.
Paunesku T, Zhang Y, Gemmell MA, Woloschak GE. p53 gene deletions in paraffin-preserved lymphoid tumors from irradiated mice. Leuk Res. 2000 Jun;24(6):511-7.
Paunesku T, Chang-Liu CM, Shearin-Jones P, Watson C, Milton J, Oryhon J, Salbego D, Milosavljevic A, Woloschak GE. Identification of genes regulated by UV/salicylic acid. Int J Radiat Biol. 2000 Feb;76(2):189-98.
 View more Publications by Gayle E. Woloschak, PhD
listed in the National Library of Medicine (PubMed) |
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