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Charles Goolsby, PhD
Patterson Professor of Pathology
Director, Flow Cytometry Laboratory
Director of Robert H. Lurie Comprehensive Cancer Center/
Northwestern University Flow Cytometry Core Facility
Olson 2-456
303 E. Chicago Avenue
Chicago, IL 60611
c-goolsby@northwestern.edu
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Phone: (312) 926-6948
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Clinical Pathology
Flow Cytometry
Medical School
Pennsylvania State University (PhD; Biophysics)
Site of Fellowship
Los Alamos National Laboratory
Research Interests
Investigation of the basic cell biology of
B chronic lymphocytic leukemia (CLL) is the major research interest of
the laboratory. B-CLL is characterized by malignant cells having an anti-apoptotic
phenotype and the accumulation of malignant cells is thought to be primarily
driven by a block in apoptosis. However, when B-CLL cells are placed in
in vitro culture, rapid apoptosis occurs raising the question of whether
there are inherent defects in the apoptotic regulatory machinery or whether
the dysregualtion seen is due to paracrine and/or autocrine factors. We
are currently investigating potential paracrine/autocrine loops regulating
apoptosis in the malignant B cell population. Specifically, the potential
role of altered cytokine production by tumor specific oligoclonal T cells
in these patients and its relationship to apoptosis control in the malignant
B cells is being studied.
Although proliferation centers are seen in B-CLL, an
additional abnormality is dysregulation of proliferation control in the
malignant B cells with few proliferating cells seen in vivo and a lack
of proliferative response to a number of normal B cell mitogens. In fact,
although non-proliferative, the B cells in B-CLL patients may not be blocked
in G0 but rather at a later point in the cell cycle. The other major area
of investigation in the laboratory is elucidation of the mechanisms of
proliferation dysregulation in these patients.
Lastly, the laboratory is also involved in development
of a number of complex multiparametric flow cytometry based clinical analyses
both for diagnostic purposes (development of solid tumor "immunophenotyping"
panels) and for patient specific therapeutic decision/monitoring purposes
including assessment of signal transduction pathways in the setting of
kinase inhibtor therapies.
Selected Publications
Goolsby C, Paniagua M, Tallman M, Gartenhaus RB. Bcl-2 regulatory pathway is functional in chronic lymphocytic leukemia. Cytometry B Clin Cytom. 63:36-46, 2005<
Chen YH, Tallman M, Goolsby CL, Peterson LC. Immunophenotypic variations in hairy cell leukemia. Amer J Clin Path 125:1-9, 2006.
Nelson BP, Treaba D, Goolsby C, Williams S, Dewald G, Gordon L, Peterson LC. Surface immunoglobulin positive lymphoblastic leukemia in adults; a genetic spectrum. Leuk Lymphoma. 47:1352-1359, 2006.
Takeda A, Goolsby C, Yaseen NR. NUP98-HOXA9 Induces long-term proliferation and blocks differentiation of primary human CD34+ hematopoietic cells. Cancer Res. 66:6628-37, 2006.
Marti G, Orfao A, Goolsby C. ZAP-70 in CLL: towards standardization of a biomarker for patient management: history of clinical cytometry special issue. Cytometry B Clin Cytom. 70:197-200, 2006.
View more Publications by Charles Goolsby, PhD
listed in the National Library of Medicine (PubMed)
Recommended Resources
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